Demi Vollering (SD Worx-Protime) received the stage 3 ITT to take the GC lead and the yellow jersey. The 2023 general winner coated the 6.3-kilometre course by the centre of Rotterdam in 7:25 minutes, beating ITT world champion Chloé Dygert (Canyon-SRAM), Loes Adegeest (FDJ-Suez), and Cédrine Kerbaol (Ceratizit-WNT) by 5 seconds.
Charlotte Kool (DSM-Firmenich PostNL) gave her all however completed 25 seconds slower than Vollering and needed to relinquish the yellow jersey, however retains the inexperienced jersey for main the factors classification.